HYDROTHERMAL SYNTHESIS AND FABRICATION OF ZINC OXIDE FOR ENZYMATIC GLUCOSE BIOSENSOR

Abstract

An enzymatic sensor for glucose detection based on zinc oxide nanorods (ZnO NRs) modified indium tin oxide (ITO) electrode was constructed via hydrothermal process. The electrode was characterized by field-emission scanning electron microscopy (FESEM) and X-ray diffractometer. The electrochemical behavior of the produced electrodes was investigated by cyclic voltammetry. Under the optimum condition, the modified electrode displays a linear response to glucose in concentration from 0.05 to 20 mM with sensitivity of 1.602 μA/mM.cm² and a limit of detection (LOD) of 0.07 mM. The calculated Michaelis Menten constant, Km was 3.07. The lower value of Km means that the immobilized GOx hold a high enzymatic activity, and the proposed modified electrode exhibited a high affinity for glucose. This makes the system is of choice for glucose detection.