THE ANTIBACTERIAL ACTIVITY AND MODE OF ACTION OF EXPERIMENTAL INHIBITOR

Abstract

The development of antimicrobial resistance in bacteria has led to serious health problems worldwide. Therefore, the need to search for new antimicrobials is essential as it has become an urgent necessity. In this study, two types of compounds, putative cell wall inhibitors (TT225) and DNA synthesis (nitroxoline derivatives ) inibitors that were discovered via structure based drug design and modification of existing scaffolds respectively were analysed for their antimicrobial activity and mode of action. Minimum inhibitory concentration (MICs) against S.aureus and B.subtilis was determined while the mode of action studies was confirmed by using B.subtilis antibiotic biosensors. Results reveal that TT225 and 2 nitroxoline derivatives (GIS213 and GISB4) showed inhibition activity against S.aureus whereas in case of B. subtilis, 13 nitroxoline compounds showed positive results. In addition, the B.subtilis biosensors has revealed that among the nitroxoline derivatives only GISB4 and GIS249_2 were successful to induce the DNA synthesis.